PI
博士研究生导师；教授
李文生，教授，博士、留美学者，博士及博士后导师。上海爱尔眼科医院副院长及研究所所长。1993年7月昆明医学院临床医学专业本科毕业，获临床医学学士学位，1997年7昆明医学院眼科学硕士毕业（白内障专业），获临床医学硕士学位，2001年7月中南大学湘雅医学院眼科学临床型博士研究生（玻璃体视网膜病专业），获临床医学博士学位。
2001.9-2005.1 昆明医学院第二附属医院眼科 讲师，副教授，主任，硕导
2005.2-2010.6 温州医学院附属眼视光医院 教授，主任，PI，博导
2010.6-2011.12 美国麻省大学医学院 眼科及基因治疗中心 (UMMS) 研究员
2012.1-2012.11 美国国立卫生研究院（NIH）美国国家眼科研究所（NEI） 客座研究员
2013.2-2016.10 厦门大学附属厦门眼科中心教授，博导，董事长助理，集团科教部主任，GCP（筹）主任
2016.09-今 上海爱尔眼科医院副总院长，教授，博导，研究所所长，GCP主任，特需国际部主任及首席专家。
到目前为止，以第一作者或通信作者在国内外Ophthalmology（IF14.3）等著名刊物上发表专业论文120篇（其中SCI文章48篇，总IF超过150），研究结果被Nature杂志、美国最新版临床指南（Preferred Practice Pattern, PPP）引用；主编《循证眼科学》等5部专著，参编8部著作，获9项有重要影响的国内外奖励，主持19项包括国家重大专项、国家863计划、国家自然科学基金等在内的国家、省部级科研课题，授权发明专利一项，培养博士（后）、硕士研究生20余人，应邀进行国内外专题讲座180余次。
由于李文生教授在专业领域所做出的突出贡献，人民日报（两次）、央视频、人民网、新华网、中国科学报、工人日报和健康报（两次）以及《中华英才》等中央和国家级媒体都进行了报道！
现任
主要研究方向：
1、视网膜变性疾病基因治疗及发病机理；
2、白内障手术及微创玻璃体手术创新技术的研究；
3、超高度近视眼治疗的新方法研究；
4、循证眼科学及眼科管理学研究等。
主要学术成果：
1、学术论文
到目前为止，以第一作者或通信作者在国内外Ophthalmology（IF14.3）等著名刊物上发表SCI文章48篇，研究结果被Nature杂志、美国最新版临床指南（Preferred Practice Pattern, PPP）引用。具体如下：
1.Yajun Wu, Yuliang Feng, Jiasong Yang, Yuwen Ran, Zongtao Shu, Xiaobo Cen, Wensheng Li*. Anatomical and Micro-CT measurement analysis of ocular volume and intraocular volume in adult Bama Miniature Pigs, New Zealand rabbits, and Sprague-Dawley rats. PLoS One, 2024 Sep 20;19(9):e0310830.
2. Li W, Zheng Q,
Chen B, Tzekov R*. Novel
frontiers of retinal neurodegenerative diseases（Editorial）. Front Med (Lausanne), 2024 Aug 14;11:1470998.
3. Qingchen Li, Xinyi Liu,Jiasong Yang, Yumeng Dai, Wensheng
Li*. Comparison of Barrett TK
Universal II and conventional Barrett Universal II formulas in power
calculations for 3 presbyopia-correcting intraocular lenses. Clin Ophthalmol, 2024 May
25;18:1457-1465.
4. Yajun Wu, Hua Fan, Yuliang Feng, Jiasong Yang, Xiaobo
Cen, Wensheng Li*. Unveiling the gut microbiota
and metabolite profiles in guinea pigs with form deprivation myopia through 16S
rRNA gene sequencing and untargeted metabolomics. Heliyon, 2024 May 6;10(9):e30491.
5. Qingchen Li, Xiaolin Xie,Jiasong Yang, Peng Gao, Wensheng
Li*. Bilateral femtosecond
laser-assisted clear lens extraction with trifocal intraocular lens
implantation for presbyopia correction: A short-term observational study in
Chinese adults. Clin Ophthalmol, 2024 Feb 29;18:623-630.
6. Guohai Chen ,Radouil Tzekov ,Yan Fang,Yuhua Tong, Wensheng Li*. Internal limiting membrane
peeling in rhegmatogenous retinal detachment:a meta-analysis. PLOS ONE, 2024,0297230.
7. Xiaodong Chen, Radouil Tzekov, Mingyang Su, Yusheng
Zhua, Aidong Han, Wensheng Li*. Hydrogen
peroxide-induced oxidative damage and protective role of peroxiredoxin 6
protein via EGFR/ERK signaling pathway in RPE cells. Front Aging Neurosci,2023
DOI 10.3389/fnagi.2023.1169211.
8. Xue Wang, Xiaomei Liu, Radouil Tzekov, Chaofeng Yu,
Jiasong Yang, Yuliang Feng, Yajun Wu, Yali Xu, Shiying, Wensheng
Li*. Fenofibrate ameliorates RPE injury induced by excessive
fat through upregulation of PI3K/AKT signaling. Drug Des Devel Ther, 2023;21:17:3439-3452.
9. Yajun Wu, Yuliang Feng, Jiasong Yang, Xue Wang, Hua
Fan, Zitong Yu, Xiaolin Xie, Yumeng Dai, Wensheng Li*.Different approaches for the treatment of myopic
choroidal neovascular: a network meta-analysis. Int J Ophthalmol, 2023 Dec 18;16(12):2105-2116.
10. Hua Fan, Mingming Zhang, Radouil Tzekov, Zhuyun Qian,
Jiasong Yang, Wensheng Li*. Postoperative
Outcome of Combined Phacovitrectomy in Eyes with Excessive Myopia (> -30
dpt). Case Rep Ophthalmol Med, 2023
Nov 14;2023:7367922.
11. Yajun Wu, Yuliang Feng, Jiasong Yang, Hua Fan, Zitong
Yu, Xiaolin Xie, Yumeng Dai, Xin Huang*, Wensheng Li*. Effects of exogenous retinoic acid on ocular parameters
in guinea pigs with form deprivation myopia Effect of RA on FDM. Front Cell Dev Biol,2023
Mar 17;11:1160897.
12. Yajun Wu, Xiangdong Luo, Yuliang Feng, Jiasong
Yang, Hua Fan, Wensheng Li*. Comparison of the accuracy of axial length measurement
by different imaging methods in Sprague Dawley rats. Front
Neurosci,2023 Jan 6;16:1106904.
13. Xue Wang, Chaofeng Yu, Xiaomei Liu, Jiasong Yang,
Yuliang Feng, Yajun Wu, Yali Xu, Yihua Zhu*, Wensheng Li*. Fenofibrate ameliorated systemic and retinal
inflammation and modulated gut microbiota in high-fat diet-induced mice. Front Cell
Infect Microbiol,2022;12:839592.
14. Guohai Chen,
Radouil Tzekov, Wensheng Li*,
Fangzheng Jiang, Sihong Mao, Yuhua Tong.Intravitreal conbercept as an adjuvant
in vitrectomy for proliferative diabetic retinopathy: a meta-analysis of
randomised controlled trials.Eye,2022;36(3):619-626.
15. Xue Wang, Radouil T. Tzekov, Chaofeng Yu, Yihua Zhu,*
Wensheng Li*.Comparsion of efficacy and
safety between conbercept and ranbizumab in neovascular age-related macular
degeneration: a meta-analysis of randomized controlled trials.Ophthalmic
Research, 2022;65(2):140-151.
16. Guohai Chen, Sihong Mao, Yuhua Tong, Fangzheng Jiang, Wensheng Li*. Fovea sparing versus complete internal limiting
membrane peeling for myopic traction maculopathy: a meta- analysis. Int
Ophthalmol, 2022;42(3):765-773.
17. Hua Fan, Zhuyun Qian, Radouil T. Tzekov, Dong Lin,
Hongxia Wang, Shibo Tang, Wensheng Li*. A new two-step anesthesia for
microincision vitrectomy surgery： a prospective, randomized clinical
trial. Ophthalmic Res, 2021;64(1):34-42.
doi: 10.1159/000508510.
18. Xue Wang, Liying Tang, Zhaoqiang Zhang, Wensheng Li*, Yongxiong Chen*. Keratocytes
Promote Corneal Neovascularization through VEGFr3 induced by PPARα-inhibition. Exp Eye Res, 2020;
21:107982.
19. Guohai Chen, Radouil Tzekov, Wensheng Li*,
Fangzheng Jiang, Sihong Mao, Yuhua Tong. Inverted ILM Flap Technique versus
Conventional ILM Peeling for Idiopathic Large Macular Holes: A Meta-Analysis of
Randomized Controlled Trials. PLOS ONE, 2020,15(7):e0236431.
20. Xue Wang, Chaofeng Yu, Radouil Tzekov, Yihua Zhu, Wensheng Li*. The effect of human gene therapy for RPE65-associated
Leber congenital amaurosis on visual function: A systematic review and
meta-analysis. Orphanet Journal of Rare Disease, 2020;15:49.
21. Jinjing Cao, Xiaomei Liu, Yun Yuan, Feifei Wang, Wen
Kong, Guohua Shi, Wensheng Li*, Chun Zhang.A
rAAV2/6 Mutant with Enhanced Targeting for Mouse Retinal Müller Cells. Curr Eye Res, 2020;45(1):64-71.
22. Guohai Chen, Radouil Tzekov, Wensheng Li*,
Fangzheng Jiang, Sihong Mao, Yuhua Tong. Incidence of endophthalmitis after
vitrectomy: a systematic review and a meta-analysis. Retina,
2019;39(5):844-852.
23. Guohai
Chen, Radouil Tzekov, Wensheng Li*, Fangzheng Jiang, Sihong Mao, Yuhua
Tong. Iatrogenic retinal breaks and postoperative retinal detachments in
microincision vitrectomy surgery compared with conventional 20-gauge
vitrectomy: a meta-analysis. Eye, 2019;33(5):785-795.
24. Zhuyun Qian, Hongxia Wang; Hua Fan; Dong Lin; Wensheng Li*. Three-dimensional digital visualization for
phacoemulsification and IOL implantation. India J Ophthalmol, 2019;67(3):341-343.
25. Zhuyun Qian, Hua Fan, Yong Tao*, Wensheng Li, Wei Gu. Herpetic
Anterior Uveitis in a Chinese Referral Center: Clinical Manifestations and
Laboratory Test Results. Ocul Immunol Inflamm，2019：1:1-6.
26. Zhuyun Qian, Haili Li, Yong Tao*, Wensheng Li*. Initial
intravitreal injection of high-dose ganciclovir for Cytomegalovirus Retinitis
in HIV-negative Patients. BMC Ophthalmology, 2018, 18:314.https://doi.org/10.1186/s12886-018-0983-z.
27. Xiaodong Chen, Mingyang Su,Taotao Chen,Haiyan Hong,
Aidong Han, Wensheng Li*. Oxidative stress affects retinal pigment epithelium
cells survival through epidermal growth factor receptor/AKT signaling pathway. Int J
Ophthalmol, 2017;10(4):507-514.
28. Guohai Chen, Radouil Tzekov, Wensheng Li*, Fangzheng Jiang, Sihong Mao, Yuhua Tong. Subfoveal
Choroidal Thickness in Central Serous Chorioretinopathy: A Meta-Analysis. PLoS ONE, 2017;12(1):e0169152.
29. Xiaodong Chen, Radouil Tzekov, Mingyang Su, Haiyan
Hong, Aidong Han, Wensheng Li*.
Auranofin inhibits retinal pigment epithelium cell survival through reactive
oxygen species-dependent EGFR/MAPK signaling pathway. PLoS ONE,2016;11(11):e0166386.
30. Guohai Chen, Radouil Tzekov, Wensheng Li*, Fangzheng Jiang, Sihong Mao, Yuhua Tong.Deep Anterior
Lamellar Keratoplasty versus Penetrating Keratoplasty: A Meta-Analysis of
Randomized Controlled Trials.Cornea,2016,35(2):169-74.
31. Guohai Chen, Radouil Tzekov, Wensheng Li*, Fangzheng Jiang, Sihong Mao, Yuhua Tong. Subthreshold
Micropulse Diode Laser versus Conventional Laser Photocoagulation for Diabetic
Macular Edema: A Meta-Analysis of Randomized Controlled Trials. Retina, 2016,36(11):2059-2065.
32. Guohai Chen, Radouil Tzekov, Wensheng Li*, Fangzheng Jiang, Sihong Mao, Yuhua Tong.
Pharmacogenetics of Complement Factor H Y402H Polymorphism and Treatment of
Neovascular Age-Related Macular Degeneration with Anti-Vascular Endothelial
Growth Factor Agents: A Meta-Analysis. Scientific Reports,2015,5:14517.
33. Qinxiang Zheng, Yueping Ren, Radouil Tzekov,Shanshan
Hua, Minghan Li, Jia Qu, Wensheng Li*.ITRAQ
based proteomic analysis of visual cycle associated proteins in RPE of rd12
mice before and after RPE65 gene delivery. Journal of Ophthalmology,2015, ID 918473, 8 pages.
34. Guohai Chen, Wensheng Li*, Radouil Tzekov, Fangzheng Jiang, Sihong Mao, Yuhua
Tong. Bevacizumab versus Ranibizumab for Neovascular Age-Related Macular
Degeneration: A Meta-Analysis of Randomized Controlled Clinical Trials. Retina,2015;35(2):187-93.
35. Guohai Chen, Wensheng Li*, Radouil Tzekov, Fangzheng Jiang, Sihong Mao, Yuhua
Tong. Ranibizumab Monotherapy or Combined with Laser versus Laser Monotherapy
for Diabetic Macular Edema: A Meta-Analysis of Randomized Controlled Trials. PLoS ONE,2014;0115797.
36. Guohai Chen, Wensheng Li*, Fangzheng Jiang, Sihong Mao, Yuhua Tong.Ex-PRESS
Implantation Versus Trabeculectomy in Open-Angle Glaucoma: A Meta-Analysis of
Randomized Controlled Clinical Trials. PLoS ONE,2014;9(1):e86045.
37. Qinxiang Zheng, Ying Zhang, Yueping Ren1, Yijun Wu,
Shelley Yang, Yuanping Zhang, Hao Chen, Wensheng Li*, Yihua Zhu*.Anti-proliferative and apoptotic effects of
indomethacin on human retinoblastoma cell line Y79 and the involvement of
β-catenin, NF-kB and Akt signaling pathways. Ophthalmic Research, 2014;51(2):109-115.
38. Qinxiang Zheng, Ronghan Wu, Shelley Yang, Yuanping
Zhang, Ying Zhang, Wensheng Li*. Clear
lens phacoemulsification combined with vitrectomy to correct high myopia- four
years follow-up. Ophthalmic Research,2013;49(2):73-80.
39. Qinxiang Zheng, Yueping Ren, Radouil Tzekov, Yuanping
Zhang, Bo Chen, Jiangping Hou, Chunhui Zhao, Jiali Zhu, Ying Zhang, Xufeng Dai,
Shan Ma, Jia Li, Jijing Pang, Jia Qu, Wensheng Li*. Differential Proteomics and Functional Research
Following Gene Therapy in a Mouse Model of Leber Congenital Amaurosis. PLoS ONE, 2012,7(8): e44855.
40. Qinxiang Zheng, Shelley Yang, Yuanping Zhang,Ronghan
Wu, Jijing Pang, Wensheng Li*. Vitreous
surgery for macular hole-related retinal detachment after phacoemulsification
cataract extraction: Ten-year retrospective review. Eye,
2012;26(8):1058-1064.
41. Qin-Xiang Zheng, Rong-Han Wu, Yuan-Ping Zhang,
Ming-Xu, Wen-Sheng
Li*.Anterior segment complications after phacoemulsification
combined vitrectomy and foldable intraocular lens implantation. Int J
Ophthalmol, 2010, 3 (3): 249-254.
42. Wensheng Li, Yune
Zhao, Peijian Miao, Qinxiang Zheng, Ronghan Wu, Jingwei Zheng, Bo Zheng, Jia
Qu. Phacoemulsification complication. Ophthalmology, 2010,117(6):1275.e1-3.
43. Wensheng Li, Gang
Sun, Qinxiang Zheng, Ronghan Wu.Cataract phacoemulsification surgery in
105-year-old patients.J of Cataract & Refract Surg,2010,36(4):691-692.
44. Wensheng Li, Ronghan
Wu, Xindan Wang,Ming Xu, Gang Sun,Changwen Sun.Clinical results of Densiron 68
intraocular tamponade for complicated retinal detachment. Ophthalmologica,2010;224(6):354-360.
45. Wensheng Li, Jingwei
Zheng, Qinxiang Zheng, Ronghan Wu, Xindan Wang, Ming Xu.Clinical complications
of Densiron 68 intraocular tamponade for complicated retinal detachment. Eye, 2010, 24(1):21-28.
46. Wensheng Li, Gang Sun, Ronghan
Wu, Xindan Wang, Ming Xu, Changwen Sun.Longterm results after phacovitrectomy
and foldable intraocular lens implantation. Acta Ophthalmol, 2009:87(8): 896–900.
47. Wensheng Li, Ronghan
Wu, Xindan Wang,Ming Xu, Gang Sun,Changwen Sun.Clinical complications of
combined phacoemulsification and vitrectomy for eyes with co-existing cataract
and vitreoretinal diseases. Eur J Ophthalmol, 2009; 19 (1) : 37 - 45
48. Wensheng Li, Fansheng Kong, Xia Li, Xufeng Dai, Xiaoqiang Liu, Ronghan Wu, Xiangtian Zhou, Fan. Lü, Jia Qu*, Bo Chang, Qiuhong Li, W.W. Hauswirth, Ji-jing Pang*.Gene therapy following subretinal AAV5 vector delivery is not affected by a previous intravitreal AAV5 vector administration in the partner eye. Mol Vis,2009;15:267-275.
2、主持课题
1.Peroxiredoxin6蛋白相关信号通路在RPE细胞氧化损伤中的调控机制研究.国家自然科学基金面上项目；
2.Peroxiredoxin 6蛋白对氧化损伤的ARPE-19细胞内P13KAKt的调控机理研究.人社部留学人员择优资助项目；
3.Peroxiredoxin在rd12鼠基因治疗过程中的动态变化及作用机制研究.教育部高等学校博士学科点专项科研基金；
4.新型AAV介导CYP4V2基因治疗结晶样视网膜色素变性.湖南省自然科学基金；
5.不同玻璃体切除手术方式治疗高度近视性黄斑劈裂的前瞻性多中心研究. 湖南省技术创新引导计划；
6.PRDX6调控对RPE细胞氧化应激损伤中EGFR信号通路的作用. 中国博士后科学基金；
7.肠道菌群在近视中的作用及机制研究.爱尔眼科研究所临床—基础联合PI基金；
8.NSAIDs 缓释剂制备筛选及对眼内炎症动物模型的抑制作用研究.爱尔眼科研究所临床— 基础联合 PI基金；
9.PPARα基因介导的脂肪酸代谢在肠道-视网膜轴中的作用机制研究.爱尔眼科研究所临床— 基础联合 PI基金；
10.一项前瞻性的，观察性的研究观察PanOptix植入不同眼底特征META-PM分期的高度近视患者的术后视觉结果.爱尔康基金；
11.两种玻璃体切除手术方式治疗高度近视黄斑劈裂的前瞻性对照研究.爱尔眼科医院集团.临床研究所科研基金项目；
12.不同玻璃体切除手术方式治疗高度近视性黄斑劈裂的前瞻性多中心研究.爱尔眼科医院集团；
13.厦门市眼底病重点专科建设项目. 厦门市卫生计生委；
14.Peroxiredoxin 6蛋白对氧化损伤的ARPE-19细胞内KEAP1-NRF2-ARE的调控机理研究.教育部留学回国人员科研启动基金；
15.Peroxiredoxin 6蛋白相关信号通路在ARPE-19细胞氧化损伤中调控机制.厦门市科技计划；
16.Peroxiredoxin 6蛋白对氧化损伤的ARPE-19细胞内KEAP1-NRF2-ARE的调控机理研究.福建省卫生厅；
17.利用AAV载体进行遗传视网膜疾病基因治疗的临床前研究.国家科技重大专项分课题；
18.肿瘤基因治疗新技术开发与治疗产品研发.国家863计划重点项目一级子课题；
19.玻璃体视网膜手术联合白内障手术研究.卫生部科教基金；
20.增生性玻璃体视网膜病变蛋白质组的生物信息学研究.浙江省自然科学基金；
21.遗传视网膜疾病基因治疗研究.温州医科大学重大项目；
22.双手微小切口白内障超声乳化和25G微创玻璃体切割联合手术研究.温州医科大学重大项目；
23.增生性玻璃体视网膜病变的蛋白质组学动态研究.中山大学中山眼科中心眼科学实验室开放课题；
24.吲哚美辛防治增殖性玻璃体视网膜病变的实验研究. 云南省教育厅科学研究基金重点项目；
25.神经生长因子保护视网膜色素上皮细胞凋亡的分子机理.云南省教育厅科学研究基金重点项目。
3、主要出版著作
1.《循证眼科学》，人民卫生出版社，三主编之一，2020。
2.《眼底光学相干断层扫描学习精要》，科学出版社，编委，2017。
3.《眼科学学习指导及习题集》（8年制《眼科学》配套教材），人民卫生电子音像出版社，编委，2016。
4.《眼科学实习指导》（8年制《眼科学》配套教材），人民卫生电子音像出版社，编委，2016。
5.《多媒体课件与在线课堂》（8年制《眼科学》配套教材），人民卫生电子音像出版社，主编，2015。
6.八年制《眼科学》教材，人民卫生出版社，编委，2015。
7.《Complications of Vitreoretinal Surgery》，Lippincott Williams & Wilkins，编委，2013。
8.《10000个科学难题》医学卷，科学出版社，编者，2011。
8.《玻璃体切除联合白内障超声乳化手术》，人民卫生电子音像出版社（视听教材），主编，2009。
9.屈光性晶状体手术学》，科学出版社，编委，2009。
10.《眼科临床安全行医指南》，人民卫生出版社，主编，2007。
11.《循证眼科学》，人民军医出版社，主编，2006。
12.《新生血管性眼病》，人民军医出版社，编委，2006。